The source of this information is a 1553 letter addressed to Saint Ignatius, available here (Márgenes literarios del juego: una poética del naipe siglos XVI-XVIII, By Jean-Pierre Etienvre, p.58).Rosa Helena Chinchilla wrote:During her two years residence in Portugal, Juana depended on her
principal religious advisor, Francis Borgia, a Jesuit, who became the most
important influence on the young princess. M. Bataillon’s description of the
religious atmosphere in the Princess’ Portuguese court reveals that Juana re-
quested twice daily visits from Borgia for religious guidance. This severe
daily regiment suggests an extreme devotional attitude of prayer common to
a movement associated with the recogidos. Borgia also devised moral games
to teach and entertain Juana’s courtiers, for example card games depicting
virtues and vices.
In the same book, at p.62, there is an interesting poem titled "Romance del juego del trunfo". My Spanish is not great, but I cannot resist translating the first lines
Hombre I Dios juegan al trunfo,
cielo i mundo es la baraja,
ponense ambos a la mesa,
i Dios reparte las cartas.
Man and God play “trunfo”,
the sky and the world are the deck,
they both seat at the table,
and God deals the cards.
(from what I understand, trunfo is a Spanish game played with a regular deck of four suits / 40 cards).